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berg M, Jaakkola MS, Woodward A, Peruga A, Prss-Ustn A (2011) Worldwide burden of disease from exposure to second-hand smoke: a retrospective analysis of data from 192 countries

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To determine the levels of mRNA expression, the first-strand cDNA was amplified using KAPA SYBR FAST Universal (KAPA Biosystems) on a BioRad CFX384 Real-Time System, BioRad, California, USA

Peel the band off and discard it in the trash instead of letting it burn up in the ashtray